Gene description for Hspa4
Gene name heat shock protein 4
Gene symbol Hspa4
Other names/aliases 70kDa
APG-2
Hsp110
Hsp70RY
mKIAA4025
Species Mus musculus
 Experiment description of studies that identified Hspa4 in Epididymal epithelial cells

1
Experiment ID1994
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34188787   
OrganismMus musculus
Experiment descriptionArrdc4-dependent extracellular vesicle biogenesis is required for sperm maturation
AuthorsFoot NJ, Gonzalez MB, Gembus K, Fonseka P, Sandow JJ, Nguyen TT, Tran D, Webb AI, Mathivanan S, Robker RL, Kumar S.
Journal name J Extracell Vesicles
Publication year2021
SampleEpididymal epithelial cells
Sample nameCBA/CaH male mice
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectometry
EV-TRACK -

2
Experiment ID1994
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34188787   
OrganismMus musculus
Experiment descriptionArrdc4-dependent extracellular vesicle biogenesis is required for sperm maturation
AuthorsFoot NJ, Gonzalez MB, Gembus K, Fonseka P, Sandow JJ, Nguyen TT, Tran D, Webb AI, Mathivanan S, Robker RL, Kumar S.
Journal name J Extracell Vesicles
Publication year2021
SampleEpididymal epithelial cells
Sample nameCBA/CaH male mice
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectometry
EV-TRACK -

3
Experiment ID1995
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34188787   
OrganismMus musculus
Experiment descriptionArrdc4-dependent extracellular vesicle biogenesis is required for sperm maturation
AuthorsFoot NJ, Gonzalez MB, Gembus K, Fonseka P, Sandow JJ, Nguyen TT, Tran D, Webb AI, Mathivanan S, Robker RL, Kumar S.
Journal name J Extracell Vesicles
Publication year2021
SampleEpididymal epithelial cells
Sample nameCBA/CaH male mice
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectometry
EV-TRACK -

4
Experiment ID1995
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34188787   
OrganismMus musculus
Experiment descriptionArrdc4-dependent extracellular vesicle biogenesis is required for sperm maturation
AuthorsFoot NJ, Gonzalez MB, Gembus K, Fonseka P, Sandow JJ, Nguyen TT, Tran D, Webb AI, Mathivanan S, Robker RL, Kumar S.
Journal name J Extracell Vesicles
Publication year2021
SampleEpididymal epithelial cells
Sample nameCBA/CaH male mice
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectometry
EV-TRACK -

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